This is the first time since Village of Sonans!? (2000), 689 episodes earlier, that a Wobbuffet other than Jessie's has appeared.
The scene where Jessie's Wobbuffet and Dr. White's Wobbuffet share food and accidentally kiss is likely a reference to a famous scene in the 1955 animated film Lady and the Tramp (1955).
Due to Wobbuffet's absence, Pumpkaboo and Inkay recite their names in the end of Team Rocket's motto.
At one point, Meowth makes a callback to his relationship with Meowzie (and, to a lesser extent, May's Skitty, Mamie's Glameow, and Misha's Purrloin) when he tells James about his troubles with love in the past.
This is the first appearance of a female Wobbuffet in the anime (outside of Professor Oak's lectures in the Japanese version). As a result, by gender difference, Jessie's Wobbuffet is officially confirmed to be male in this episode, as he was introduced before the introduction of gender differences.