Blooper

When Austin writes XXVII on the chalkboard, the lines on the V and the I are broken in the closeup and the line over the II extends over the X's. When the camera view changes the XXVII on the chalkboard are written in a completely different style and no broken lines.

Just before Austin writes the Roman numerals on the chalk board, you can see the faded lines of "X" and "I" in the very place Austin is about to write, indicating that the set of Roman numerals was written there prior and erased, likely during scene blocking or an outtake.

When Emma is first introduced (in the hallway outside the morgue), she is wearing hosiery. When she walks into the morgue, her legs are bare.

After Jane Doe's body is brought in to the morgue on a trolley/gurney, Emma leaves. The exterior shot showing Emma's car drive away shows the drive was blocked. There is no room for an ambulance or any other vehicle to have passed Emma's car, nor is there enough room to have pushed a gurney up the drive to the house.

In the final scene, where the police are in the lab, the numbers XXVII on the board are written again the same way as their were in the first place, before they changed between shots.

Jimson Weed, Datura stramonium, is believed to have originated in Mexico, not New England, and can be found throughout the United States, (including the South and West,) and the rest of the world. The plant also has no paralytic properties, though ingesting it can cause seizures, strong hallucinations, delirium, tachycardia, hyperthermia (high body temperature), mydriasis (dilated pupils), bizarre behavior, and death.

When Tommy is providing what he believes is Jane Doe's backstory or history, he mistakenly says she was one of the victims at the Salem Witch Trials, and continues by saying the victims were all young girls. The young girls at the Witch Trials were the instigators and not the victims as Tommy states. In fact, it would have been the reverse of what he describes: Jane Doe would have been in the group of adults who were persecuted by the young girls.

When Tommy changes the stations on that radio cassette player, the dial is visible, it has long waves. That kind of radio receiver is not sold in United States, but in Europe and parts of Asia.

On the blackboard it says "peat under the finger nails" Later it's shown spelled correctly, then wrong again.

At two points in the film, places are referenced implying the movie takes place in Virginia: Norfolk in the 1st act and Virginia Commonwealth University (or VCU) in the 3rd. However, license plates on the vehicles are blue and yellow, similar to those in Pennsylvania.

When dad is first attacked by an entity in the hallway, son finds him with his right side bruised. Throughout the rest of the movie, it is the father's left side that continues to worsen. Actually, it was his left side that was injured initially. Even though the scene is dark, the audience sees it reflected in the bathroom mirror. This confused some viewers.

Why weren't the other three bodies from Jane Doe's crime scene brought to Tommy for autopsy? There would need to be forensic autopsies for all the deaths. Actually, the Sheriff clarified that because the Jane Doe couldn't be explained, her forensic autopsy was a priority to be done overnight. The others could wait until business hours the next day (or even possibly go to a different coroner).

Tommy describes the last movie he saw in the cinema: "she has Alzheimer's, he builds a house for her". His son identifies that as Le pagine della nostra vita (2004), but that story-line doesn't match his description, except for the Alzheimer's part. A more likely match would be Mike McGowan's Resta con me (2012) with James Cromwell and Geneviève Bujold; possibly an honest mistake on the son's part. Actually, that is the story of The Notebook. He builds her a house in the past while she has dementia in the present. Also, The Notebook was a wildly popular movie, so is more likely to have been the one being remembered.

When Tommy and Austin autopsy the burn victim early in the movie, Tommy quizzes his son as to the cause of death. Austin gets it wrong and Tommy points out a clue his son has missed--a crack in the skull that indicates a brain bleed, showing the man had hit his head and blacked out in the fire. Yet this supposed "mystery" would certainly have come out during the autopsy; it is unfathomable that the coroner would not note the skull fracture upon noticing it and enter it into the audio or written record along with the corresponding evidence of the enlarged brain.

At around 41 minutes, when Austin writes XXVII on blackboard, it is possible to see faint traces of these numbers on the board suggesting that they had already been written there and later erased. Austin is writing almost exactly on them.

The tooth they find in the stomach is a bicuspid, the missing tooth in the mouth is a molar. They are incorrect is saying this is the missing tooth.

When the deputy is driving away with Jane Doe's body, the van he's driving has a console stick shift and the speedometer is clearly label in K/H and not MPH. If this were in the US, as is depicted, new vans are not available with a console shift manual transmission and MPH is the standard for speed instrumentation.

"Nasal" is spelled "Nasul" on the chalkboard in the information for the previous autopsy.

Corsets that functioned to narrow the waist to noticeable extremes were not used until the Victorian Era. During the 17th century, women wore stays that mainly functioned to support the breasts and to provide a foundation for heavy garments that were typical of the era to drape upon. Tight-lacing that was seen during the Victorian Era would not be possible in these garments, as the holes for the laces were hand-sewn, and would tear if under too much strain.

Jane Doe has supernatural fire resistance when Austin and Tommy try to incinerate her body, propelling the flames towards the ceiling and attempting to kill the two coroners. Yet, she didn't have the same power to "fight back" and prevent the prior excision and organ removal.

The strychnine poisoning case is crudely mislabeled/misspelled on a morgue drawer as strychlinine.