Both James Kirk, a guest star in this episode and Jensen Ackles starred in the TV show Dark Angel (2000). They played the same character, Ben. James played the younger version, Jensen the older version.
Lindy Booth starred in the movie La próxima víctima (2005) with Jared Padalecki in 2005. Also in the cast was Jared's former fiancee Sandra McCoy.
Sam calls Dean at the chastity group leader's house and Dean hangs up without answering, saying, "Not now, Kato." He could be quoting Inspector Jacques Clouseau from the 1960's Pink Panther movie series or the Green Hornet's faithful man servant.
Lindy Booth (Vesta) starred in The Librarians (2014-2018). Christian Kane also starred in The Librarians and guest starred in an episode of Supernatural in Season 15.