Reptile House was filmed in two locations: the home of Reptile House director Tristan Risk and the TCS reptiles, and secondly at the house and garage owned by producer David Aboussafy after it was emptied prior to being sold.
Producer David Aboussafy and director/actor Tristan Risk had previously worked together on the films Anxiety (2020), Amazon Hot Box (2018), AlphaMem (2018), Odissea della Morte (2018), Ayla (2017), and Frankenstein Created Bikers (2016).