The window next to the door in Luz's house changes between shots. In the exterior shot it's covered with cardboard or some other brown sheet (we even see the explosive bounce and fall to the ground) while from the interior it's a normal window with curtains.
On the building, when Machete is preparing to shoot the Senator the back cover of the scope is seen up, but when he looks trough it, it is down.
In the opening scene where Machete and his partner are attacking the drug-house, several bullets are fired through the windscreen as they approach the building. However, when the car crashes through the building, the windscreen is completely intact and bullet-hole-less.
When Booth is driving to pick up Machete at the day labor site, he is seen driving a Mercedes Benz S-Class (W220 1999-2006). At the day labor site, he is seen in a Mercedes Benz S-Class (W116 1972-1980). When he drives away, the car switches back to the W220 model.
In addition, in the inboard scene at 0:16:36 you can see the headrest of the W116 again. Later on (e.g. when Michael Booth gets his daughter from the house), the headrest is one of a W220 model.
During final fight sequence, When Machete put machine gun on his motorbike, it has no bullet belt. But when he fire, bullet belt is attached. Again when he stops, there is no bullet belt.
In Spanish, the singular form of Federales is Federal, not Federale as constantly seen in the movie. Although this is a common Anglo misconception, it is presented on Machete's official personnel files which would never make such a mistake.
(Deliberate by filmmaker) During the pool scene, as April enters the water is played by Lindsay Lohan. After Machete enters the pool, the character is clearly not Lohan. This is because the footage is actually from the "Machete" fake trailer that preceded Robert Rodriguez's Planet Terror (2007) segment in Grindhouse (2007) - one of several pieces of trailer footage that were re-used in the movie.
In the hospital when booths thugs shoot the rolling cart in the hallway, it rolls by them and there is clearly no one under it. Then Machete is seen getting out from under the cart.
When the Padre is in the shootout with Osiris and his men, it's clear from the camera's angle that while the three men are looking for the Padre, and right before the Padre shoots two of thugs, Osiris can clearly see the whole side of the Padre hiding behind the column.
The pool scene is not filmed from a fixed position even though April used a tripod.
During the shootout at the church, Osiris Amanpour is holding a gun that tends to shoot like an automatic rifle (eg M-16). However this gun is a USAS-12 automatic shotgun with a 10-round-capacity box magazine. You can see shotgun shells ejected while watching in slow motion.
When Sartana finds Machete's computer profile it states his height as 5' 11". IRL, Danny Trejo is under 5' 6".
When Michael Booth enters the house and starts shooting everyone with a silenced gun, the first man's head gets shot before we hear the sound of a gun.