In the asylum, Lawrence is dunked in a large ice tank. When he's brought back to the surface, he has two chucks of ice on him that fall off leaving none, but when the scene cuts to him being raised and locked upright, suddenly there's a chunk of ice on his right arm.
When Aberline starts his interview with Lawrence, Aberline holds his glass with his hand covering the top and his fingers pointed down. When he sits down on the sofa, his hand is now wrapped around the side.
The language spoken by the Romani (gypsies) in the film is actually Romanian. In reality, the Romani language is completely different from Romanian. It is actually more similar to Hindi, and has many various dialects depending on the area of origin.
The steam tram the wolf man attacks has passengers riding on it. Steam tram passengers rode in a trailer coach, as the trams themselves were too smoky and noisy for passenger occupation (think Toby and Henrietta from "Thomas the Tank Engine.")
As Talbot walks the moor the moon arcs across the sky (i.e., time passing), but the sunlight on the face of it does not change at all.
In Gwen's antique shop there is what seems to be a small replica of Auguste Rodin's "The Thinker," which was first created around 1902. However, upon closer inspection this statue, while sharing the same position as the Thinker, is dressed and notably less muscular than Rodin's statue. The statue is actually a replica of Michelangelo's sculpture of Lorenzo de' Medici from his tomb.
When Lawrence Talbot is walking back to Talbot Hall, he passes a tablet that states it is 16 miles to Blackmoor. In 1891, the metric system was not yet popular in Europe. Even in 2014, much of Britain still holds out on the metric system in favour of imperial measurements (miles, yards etc.) for distances.
When Sir John Talbot is blowing out candles, they are clearly filament lamps, as the third candle lamp actually goes out before he blows on it.
In the Max Von Sydow scene (directors cut), Lawrence is sitting alone in the train cabin, all the seats are empty except for Lawrence. He dozes off and drops a paper and photo then cut to the paper and photo on the ground and Max's feet, the camera pans up to show Max sitting in the middle seat that was empty a moment ago.
One of the topiary animals is a mountain gorilla. These great apes were unknown outside of Rwanda and its environs until 1902.
When Talbot is first in Gwen Conliffe's shop in London. There is a vase of flowers containing Stargazer lilies. The Stargazer lily was created in 1978 by Leslie Woodriff.
Tower Bridge, which opened in 1894, is seen operating during the film, which is set in 1891.
When Talbot enters Gwen Conliffe's shop, we see that the door has a flower design inspired by Charles Rennie Mackintosh after 1896.
There is no evidence in the film that werewolves can distinguish blood relatives, or that such a distinction would hold any value for them while in that state. It takes a huge effort for Gwen to break through to Wolfman Lawrence, and it doesn't take. Yet when young Lawrence stumbles on his werewolf father just after he's failed to recognize his wife and brutally murdered her - his father somehow avoids attacking him.
Sir John reveals that he got the curse after getting bitten by a feral wolf boy when he was a young man but somehow the curse wasn't passed on to Lawrence or his brother John when they were born.
Gwen regrets getting Lawrence involved, saying that if she had never sent him that letter, he would still be in New York. She sent Lawrence a letter when Ben went missing in the theatrical version, but in the extended cut she went to tell him in person instead. Yet the "letter" line is retained in both versions even though it doesn't make sense in the latter.
Sir John describes St. Columbanus as a gypsy from the East, but in fact St. Columbanus was Irish and founded one of the first monasteries in Scotland (on Iona island), one of the most important in Christendom.