(at around 1h 28 mins) In the car chase scene, Zep is seen driving a late-1980s Ford Bronco II. When he arrives at the warehouse, the truck has changed to an early 1990s Ford F-150.
The stains on Adam's white T-Shirt from the toilet disappear and reappear throughout the film.
(at around 1h 14 mins) When Lawrence is in the parking lot, he is seen pinching his nose when the photo is taken; when we see the photo, he has covered his eyes completely.
(at around 1h 29 mins) When Lawrence is using the box to try to reach the phone, he gets angry and tosses it to his left side. When we see his right side, the box is still lying there. A few moments later, when he is tugging on his chains in frustration, we see the box next to the door, in the direction that he threw it.
(at around 1h 7 mins) When Adam first remembers what happened before he was kidnapped, we see him making his way back to his apartment after snapping photos of Lawrence. The camera Adam has slung over his shoulder changes from scene to scene. In the hallway outside the apartment, Adam has a black camera with a long lens and a mounted flash unit. As he enters the apartment, he has a silver camera that appears to have a very short lens and no mounted flash. Once inside, he has the black camera with long lens and mounted flash again.
(at around 59 mins) After Lawrence and Adam find the box with the cellphone and cigarettes, Lawrence tries to call 911. Three different tones can be heard and he says that the cellphone has been blocked from making calls. However, FCC rules require every telephone that can access the network to be able to dial 911, regardless of any reason that normal service may have been disconnected (including deactivated or blocked phones).
(at around 1h 13 mins) After Lawrence speaks on the cellphone for the first time and the line goes dead, there is a dial tone. Cellphones do not use dial tones.
(at around 12 mins) When Lawrence tells Adam to throw him the tape player, Adam is sitting on the floor. Adam throws it straight ahead of him. Lawrence catches it in an upwards direction. From the angle Adam threw the player, whilst sitting on the floor, Lawrence would have to crouch down. Also, Adam threw the player with one hand. It is more likely the player would be turning as it is in the air. Lawrence catches the player in a non-spinning motion.
(at around 38 mins) When Adam asks Lawrence if he's going to have any more kids, there is a small, white spot on the right side of the screen visible for less than a second. This is likely a single-frame anti-piracy watermark or a defect on the film. They appear several times in the film, especially in the final reels.
(at around 5 mins) In the beginning, when Adam says "This is what they do, man. They drug you, kidnap you, and next thing you know, you wake up in a bathtub and your kidneys are on eBay.", this is incorrect. Selling human organs has always been against eBay's Terms of Service. Adam is clearly being facetious in saying this, and this does not indicate that he or indeed the filmmakers believe that human organs could ever be sold on eBay.
(at around 43 mins) Close-up shots of the newspaper clippings lining the walls of Detective Tapp's apartment show headlines about the Jigsaw Killer's murders. However, the articles have nothing to do with the headlines, and the datelines have the news stories in different cities, ranging from Virginia to California.
(at around 37 mins) When Lawrence is about to leave the house, while his wife Alison is confronting him, the lack of ceiling and unfinished wooden rafters are visible in the set of their otherwise elegant home.
(at around 1h 30 mins) The cartridge Lawrence loads into the revolver has clearly been fired as the primer has an obvious depression.
(at around 47 minutes) Leigh Whannell acted as a stand-in for Ken Leung in the shot where Detective Sing forcefully enters the warehouse carrying a shotgun.
Lawrence was the police's initial suspect in the Jigsaw Killings. Yet it never occurred to them that these killings took a huge amount of time to plan, setup, and execute, so a surgeon who is constantly working doesn't make sense as a suspect. The devices had to be custom built, the rooms configured, and clues planed and hidden; the victims were also under surveillance for several weeks. So doing all of this required several hours. Lawrence is an oncologist, a doctor with dual specialties like this often works 90-100 hours a week easily so most of his whereabouts during the day would have been known by his coworkers. While it's possible that some of the work could have been done at night he wasn't able to survey the victims at that time, plus if he was frequently staying up all night to do all of this it would have shown in his surgical skills; his ability to perform surgery would have been severely diminished if he was suffering from sleep deprivation. It did not make sense that Lawrence was a suspect, which is something an experienced homicide detective would have realized right away.
Nothing kept Zepp from going to the police and telling them about John Kramer's crimes and telling them that John poisoned him with a slow acting poison and to be sent to the hospital. He could of also told the police that John was lying on the bathroom floor and was in the middle of observing a game.
If the only way Lawrence would be let go was to kill Adam, there was no reason for him to given a saw to cut off his foot.
(at around 1h 13 mins) After the first call Lawrence gets on the cellphone, a boom mic shadow is visible on the bathroom door.
(at around 24 mins) When Detective Sing asks Lawrence to stay and listen to Amanda, he says, "I wanna know if you wouldn't mind sticking around and listening to her testimony". What Amanda was doing was giving a statement, not a testimony. A detective simply wouldn't make that mistake.
If Zepp was hiding in Diana's closet, Lawrence should have found him unless he for some reason didn't look there to reassure her no one was in her room.