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Character error
When Fred discovers the missing tilde in the writing on the side of the Mystery Machine, he misspells mañana as "manaña".
When the gang first enter the Mystery Machine, it has regular car doors. Later on, when Scooby and Shaggy guard the van, it is equipped with suicide doors.
When Paco incites the mob against Scooby and friends at the Pyramid, Alejo's clothes suddenly changed into the same colors as Luis'. Also, the angry tourists keep swapping positions.
When Alejo tries to wake up Doña Dolores when she fainted, and Alejo comforts Luis, his wristwatch on his left arm is missing.
When Chiquita was giggling at Scooby for walking into a gravestone, and laughing at the Chupacabra tripping over a pumpkin, her eyes were colored black instead of brown.
El Chupacabra was claimed by the producers as Mexico's version of Bigfoot", although such a description is grossly inaccurate, as the chupacabra described is quite different from Bigfoot.
Jorge is supposed to be Alejo and Sofia's only child, but Carolina, Sebastian, and Natalia are given the Otero name in the credits. They could be distant cousins of the family.
While in split-screen, the gang go from speaking out loud to themselves then to typing, and finally to just speaking without typing and somehow everybody seemingly knows what each other is saying. The four-way split-screen could be breaking the "fourth wall" for them. It's merely an engaging narrative device, because scenes of people typing without talking would be extremely boring.
When Doña Dolores was talking about Diego Fuente, her skirt and Velma's were switched to each other.
This movie portrays the Chupacabra as a Mexican legend in origin; however this is not accurate since the Chupacabra has its origins in Puerto Rico.
Velma incorrectly states that Teotihuacan (the pyramid complex where the gang emerges after following a secret passage from the Museum of Anthropology) was built by the Olmec civilization. Teotihuacan is thought to have been established around 200 BCE, in the highlands of Central Mexico, near Mexico City. The Olmec civilization flourished from 1500 BCE to 400 BCE in the Gulf Lowlands to the east and south.
Throughout the film, even Mexicans say "Chupacabra" as it's adapted in English. In Spanish it's spelled Chupacabras.
Fred pronounces tilde as "til-dee". The correct pronunciation is "til-duh."