Position of Orin Scrivello's hands on his first patient during the dentist song.
Audrey has blood stains around her waist when Seymour pulls her from the plant, but they disappear in the next scene. The subsequent scene was a re-shoot in which Audrey survived.
During "Skid Row" when Seymour walks down the alley to the gate and homeless people crawl it up and sing, there is a black man in a black beret, who, a few seconds later, when Seymour turns and runs out of the alley, is standing on the sidewalk with a bottle in a paper bag.
The color of the inside of the plant's mouth changes from blue/purple to red from scene to scene.
When Audrey talks to Audrey II on the phone, her left hand disappears from the receiver between shots.
Audrey is pulled face-first into Audrey II's mouth, meaning she would be face-down on her belly. But she's face-up on her back when Seymour rescues her. It is possible that she rolled around and ended up on her back during her struggle.
When Audrey II fires the gun through the glass, sparks are visible from the pyrotechnic charges.
When Seymour is using the grass mower during the 'Somewhere that's Green' dream sequence, the length of the grass he passes over does not change.
When Orin dies his right eyebrow twitches twice.
In the first shot of the "Skid Row" number, Crystal, Ronette and Chiffon are seen briefly (in ordinary clothes) before Bertice Reading appears and starts singing, then Michelle Weeks enters the screen, magically sporting a change of costume ("Sing it child!"). Viewers would have to watch very closely to see that it's not Weeks herself at the start of the shot, but a double.
Though the year is never mentioned, the calendar behind the counter matches September of 1964. However, on the radio it is briefly mentioned that the U.S. president is John F. Kennedy, who was assassinated in 1963.
The film is set in the early-'60s however at least two cars of later production are seen: the taxi that passes Seymour as he walks to the market during "Da-Doo" is a 1974 Checker, at 0:14 a 1965 Ford drives by during the same number, and a 1968 Volkswagen Beetle is seen driving past Mushnik when he leaves his store.
During the "Somewhere That's Green" number, Audrey dusts the house with a synthetic fiber static cling duster that wasn't invented until 1973, about 15 years after the movie takes place.
In the opening number, right after Ronette sings "hear the sturm und drang in the air" you hear Crystal and Chiffon sing "yeah, yeah, yeah" but their mouths don't move at all.
The first bum in "Downtown."
During the 'Downtown' sequence, just before Audrey appears when the bum sings 'subway', the lady on the extreme right turns to the beat with the other extras and trips on the cobblestones in her high heels. She continues singing the chorus with the rest of the cast, then when they all freeze she breaks character to look down at her feet to see what she tripped on.
In one shot during the "Downtown" sequence, Audrey is obviously stepping off from a standing position on cue, although she's supposed to be continuing a walk down the sidewalk.
When Audrey sings "My arm's in a cast" her arm is not, in fact, in a cast. It's in a sling but no cast.
Seymour tells the audience that he buys Audrey Jr. from the Chinese merchant for $1.95, but only places a single coin into his hand.
During "Somewhere That's Green" Audrey isn't wearing a wedding ring but Seymour is.