At 1:26:40, when Eugene plugs in his guitar, he's wearing a hat. The camera cuts to Scratch entering the room; when it goes back to Eugene, his hat is gone.
When Willie and Eugene first hitch a ride with Frances, they get into a big rusty 4-door sedan. Later when they are in Mississippi and get a ride from the boarding house, they are picked up in the same car.
Willie has money in his hands when he catches Frances sneaking out of their hotel room. Then his hands are empty before he goes to his bag to get money to give to Frances.
When Willie and Eugene buy the Telecaster at the pawn shop, they saw off a piece of metal tubing to make Eugene a "bottleneck" to play guitar with. Later, in a close-up, he uses an actual glass bottleneck.
Early in the film, Eugene puts a Robert Johnson tape into his tape player. The first track is listed as "Crossroads Blues." It should be "Cross Road"; the song title became "Crossroads" when Cream covered it in 1968.
At the beginning, the nurse gives Hoff memprogromade, a drug that does not exist.
The real bluesman Willie Brown passed away in 1952 yet the film takes place in the 80's..
At the time the movie was filmed, Julliard did not offer classes in classical guitar.
When the bartender in Crupp's bar pulls a shotgun to break up the fight, he racks the pump. The gun is a double barrel over-under shotgun that opens up at the back, not a pump-action shotgun. It would not make a pumping sound.
During the duel, Jack Butler hands his guitar to someone, takes off his jacket, then puts the guitar back on to play his "intimidation" riff before the rest of the band starts up. Jack's riff is heard before he has his right hand anywhere near the guitar.
When Eugene and Willie are drinking whiskey after Frances leaves, Eugene starts playing his Telecaster. You can clearly hear a "tremolo effect", even though the guitar is plugged straight into the amp; the "Pignose 7-100" amplifier does not have any effects.
At the end, when Eugene walks up to the stage for the guitar duel, the Nike sneakers he has worn throughout the film sound like hard-soled shoes on the wooden floor.
When Eugene duels with Steve Vai, the audience applauds his guitar skills in the background, without making a sound.