When Larry throws the little brown jug out of the railroad car, he throws it to the right side of the window but when Detective Moe gets hit by the bottle, it comes from the left.
When Detective Moe gets out of the car and starts walking towards the stolen railroad car, he is supposed to be outside walking on dirt, but the sound he makes while walking is that of a wooden soundstage floor.
When the Stooges are fighting over the skunk, Shemp trips over one of the seats and Larry says "Watch Yourself!" to him, out of character.
When Shemp says the line "Horrible, but delicious!", his mouth does not move.
At about 10:40min Camera Shadow can be seen.