(at around 1h 35 mins) Towards the end, Selene frees Michael from the examination table by shooting off the chains attached to the handcuffs that bind him to the table. In the next few shots, when Michael is yanking off the ropes that had been around his chest, the handcuffs themselves have disappeared from his wrists, including the chains.
(at around 1h 40 mins) When Kraven tells Selene, " is the one who murdered your precious family" he does so in his native Irish accent, an accent the character does not have.
(at around 47 mins) When Selene is awakening Viktor his canines are fangs just like the rest of the vampires, but through the movie both his lateral incisors and his canines are fangs instead.
(at around 4 mins) A bald man wearing a green jacket and carrying a blue backpack appears to board the subway train three times during the opening sequence. We see him in front of Michael standing next to the subway train doors; then we see him walking past Raze toward the train; and then a second later we see him walk past Raze toward the train exactly as before.
(at around 1h 22 mins) The men behind Kahn at the door to Viktor's chamber are all different men when they get into the chamber.
(at around 26 mins) The Beretta 93 series is chambered for the 9mm Parabellum and
a double-stack magazine can hold 21 cartridges. In the hallway scene, Selene nearly empties both magazines. Yet, without obviously reloading or reasonable time to do so, Selene empties the equivalent of another magazine from each gun into the floor.
(at around 9 mins) The bullets shown in the close-ups have little glass "windows" on the shell casings, and show the UV liquid or liquid silver nitrate inside (at around 38 mins). If this were a real bullet, there would be no room in the shell for gunpowder. A shell casing is ejected from an automatic weapon, not actually part of the slug/projectile as depicted in the movie.
(at around 38 mins) Silver nitrate is a clear substance and looks nothing like the quick-silvery fluid seen in the movie, even in high concentrations (though it may turn black if stored for long periods). This may have been done intentionally however.
(at around 1h 15 mins) When Michael goes through his first transformation in the police car, he has/hasn't fangs from take to take. That's because Michael is trying to fight his transformation.
(at around 1h 40 mins) When Viktor pushes Michael through the wall, as he lands in the water you can see several large pieces of the broken wall floating on the water behind him, showing them to be polystyrene.
(at around 1h 30 mins) When Kahn rolls the grenade down a shaft full of Lycans, the grenade bounces out of the puddle at the bottom. As it does this the explosion begins from within the puddle, underneath the still airborne grenade.
(at around 20 mins) Just before Raze gets the stars removed, he walks in holding someone's body over his shoulder. He is supposed to be naked because of the transformation, but you can see a small pair of black underwear.
(at around 54 mins) Just after Viktor is awakened we see a white car make a right turn. The street sign is reversed, showing that the image has been flipped.
(at around 39 mins) When Selene is firing at the bust at the beginning of the shooting gallery scene, the squib can be seen falling out of the hole in the neck and dangling by two wires.
At the 8:30 mark, when Seline jumps down into the tunnel, there is a closeup of her feet landing where you can see both hands are empty but in the cut scene to show her standing, she is taking aim with a pistol upon standing.
Lucian, despite having the variables to prolong his life via Raze's delivery of Amelia's blood, dies needlessly. It is revealed that Viktor and Amelia's blood in combination with Michael's blood could produce a uber strong hybrid. Raze even drops the blood before Lucian in the holster. Previously, Lucian injected himself with Michael's blood to complete 1/2 steps. Only needing Amelia's blood to fulfill his goal. Yet, he does not.
(at around 59 mins) Viktor is referred to as 'The oldest and strongest vampire', but if Marcus Corvinus is the first vampire, and is an elder still living in the current time in the movie, then Viktor cannot possibly be the oldest.
(at around 23 mins) Michael's address shown on the computer is misspelled. It is Laktos Joszef 39 ut. instead of Lakatos József u. 39.