- Friends with former Grey's Anatomy (2005) co-star, Katherine Heigl; he was a groomsman at her wedding to Josh Kelley.
- Dyed his hair for the role of Dr. George O'Malley on Grey's Anatomy (2005); his natural hair color is light brown.
- Raised Catholic, Knight attended Annunciation Catholic School in Minneapolis (K-8), and later, graduated from the Academy of Holy Angels High School in nearby Richfield, Minnesota.
- He was awarded the 2010 Back Stage Garland Award for Performance in a Musical for "Parade" in a Donmar Warehouse production at the Mark Taper Forum Theatre in Los Angeles, California.
- Lived in New York when he appeared on Broadway in the following shows: "It's Only a Play" (October 9, 2014 - June 7, 2015); "A Life in the Theatre" (October 12, 2010 - November 28, 2010); "Tartuffe (January 9, 2003 - February 23, 2003)", and "Noises Off" (November 1, 2001 - September 1, 2002).
- Participated in acting coach, Kent Stephen's, Actors' Gym, while studying in Minneapolis, Minnesota in the 1990s.
- He resides in Los Feliz, California (as of November 2007).
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