During the pissing match, Ash goes into her rage state and yells "In the place of a Dark Lord, you will have a queen!". This is the same line that Galadriel says in The Lord of the Rings: The Fellowship of the Ring (2001) when she is briefly tempted into evil by the One Ring.
During the pissing match, Gary yells "don't cross the streams!" to Little Cato, a joking nod to Ghostbusters (1984) where Egon said the same line about the streams of the proton packs.
After the pissing contest, AVA tells Ash "you now have a UTI". UTI stands for urinary tract infection.