Tohoru Masamune, who voices the three samurai, had previously starred as the Shredder in Teenage Mutant Ninja Turtles (2014).
Brittany Ishibashi, who voices Akemi, had previously starred as Karai in Teenage Mutant Ninja Turtles: Out of the Shadows (2016).
The episode title "Yojimbo" is a tribute to Usagi Yojimbo, and also to the samurai film Yojimbo (1961) that inspired the comic. The episode features several tributes to the film:
- Usagi scratches his head in a motion similar to Kuwabatake Sanjuro
- The lizard with a severed hand in its mouth parallels a scene from the film featuring a dog with a hand in its mouth
- Kintaro hides under the floors from samurai after him, just as Sanjuro did.
Kintaro the pug is based on a legendary Japanese folk hero Kintaro ("Kin"-"Taro", "Golden Boy"), a young boy in a red haragake (apron) who had super strength and fought with an axe.