First appearance of Agent Piper.
The tribesman at the beginning of the show had markings very similar to Black Panther.
The town that Hive buys, particularly the Main Street, was filmed in Galisteo, New Mexico, the same town used in Thor (2011).
Atypically, in this episode, there is no post-S.H.I.E.L.D. logo, pre-End Credits scene.