This is the first mention of the long running gag that "Terry loves yogurt."
Pedowski says his special little boy is going to play Lacrosse at Duke University next year. At the time, the team was involved in a sex assault scandal and implication is his son is a horrible person and possible future rapist.
Peralta has named his desk mouse Algernon; this is a subtle reference to the book, Flowers For Algernon, by Daniel Keys, where the mouse is called Algernon.
First use of the opening theme and credits sequence.
First appearance of the precinct sets constructed for the series. The pilot used different sets for the bullpen and adjacent rooms.