Carnival's posters show several members of different incarnations of the team, such Beast Boy, Dorothy Spinner, Flex Mentallo, Rhea Jones and Shasta the Living Mountain.
Batgirl's first appearance. The villains appearing in the prologue short were selected based on Batgirl's other "first" appearances. When the short pilot Batgirl (1967) was filmed, Killer Moth was the first villain she faced as well as the first villain she faced in the comics. When Batgirl actually made her first appearance on Batman (1966), the first villain she faced was the Penguin.