A poster for Twisted Souls appears on the wall of the second Train Station in the short film Woes of the Departed.
The documentary 'The Last Movie House', about the Movie House Video Rental store in Kidderminster mentions Twisted Souls and a possible sequel. The main villain in Twisted Souls, Nigel Cooper, was named after the shop's proprietor.
The cemetery in the film was actually a number of different locations. Two cemeteries, one in the town centre that was used for the gates, and an abandoned rural one for most shots. Any shots of the graves being dug up were done in a disused quarry in Kidderminster, with cardboard tombstones. While shooting a scene that involved a coffin and dummy, an old lady walking her dog spotted the filming, and phoned the Police, telling them that there were devil worshipers sacrificing someone in the Quarry. The shoot was held up when the Police arrived and everything had to be explained. Two decades later, one of the Policemen who arrived, enrolled on a video production course designed and taught by director R. N. Millward.
There were two attempts to do a special edition. Footage exists of an alternate scene when Cooper goes home, introducing the character of Alex earlier in the film, to create a better flow with the ending. Originally Cooper's niece Linda was supposed to appear at the end, but the actress became ill and a new character was substituted. The new version was designed to resolve this as much as possible. There is also footage of an alternate undertaker scene and more zombies for the ending.
The film's title was taken from Spookies, which had 'Twisted Souls Inc' as the production company in the opening titles. The filmmakers did not know at the time that Twisted Souls was actually planned to be the original title for Spookies.