The scene in which The Monarch drops his robe to reveal a tattoo on his back resembles a similar moment in Red Dragon (2002).
First appearance of the Order of the Triad (Dr. Orpheus, Jefferson Twilight, and The Alchemist).
First appearance of the Murderous Moppets (Tim-Tom and Kevin).
The scene of Dr. Venture washing the walking eye is a parody of the car washing scene from Cool Hand Luke.
When Doctor Venture is washing the walking eye Truckules and Sargent Hatred can be seen waiting in line to be an arch for the Order of the Triad