Although the hick rooster character is listed as "Clem" in credits, the hick chick clearly calls him "Lem" every time she addresses him. His full name is "Lem Kadoodlehopper". This is a parody of "Clem Kadiddlehopper", a character created by radio and TV entertainer Red Skelton.
At the barn dance, the sign above the entrance identifies the musical performers as "Spike Bones and his Chicky Slickers". This is a parody of the popular performers Spike Jones and His City Slickers.
Three characters' voices are impersonations of Hollywood stars: Katharine Hepburn (Daisy), Charles Boyer (Charles), and Mae West (the nurse).
The label of "Pre-War" on the villain's spare tire is a nod to the wartime rubber tire shortage America had just gone through.