On the side table in the foreground around 4:20 is a large bottle with a label on the left-hand portion of which the letters "VOD" are visible; the right-hand portion of the label is torn off. The animators appear to have sneaked in an alcohol-related gag -- "VODKA"! -- under the radar of the Hays Office.
One of the foods the little pig fantasizes appears to be a bacon sandwich...
The title is based on a popular short story of the same name, first published in 1905 by Ellis Parker Butler.
Spaghetti was a popular dish at the time, though other pastas were usually found in Italian neighborhoods. Most Americans were mostly familiar with spaghetti and meatballs or macaroni and cheese back then.
The evil scientist was a nod to the numerous horror and science fiction features popular at the time.