- Cites her seven minutes of screen time in the pilot of ER (1994) as the turning point in her career. Michael Crichton and Steven Spielberg had been duly impressed with her death scene, as filmed through the eyes of series boyfriend Doug Ross (George Clooney), a decision was made to 'resurrect' her character.
- Gave birth to her first child at age 41, son Kieran Lindsay Lieberthal. Child's father is her husband, Keith Lieberthal. (January 17, 2008)
- Born to Paul Margulies, an advertising writer, best known for an Alka-Seltzer catchphrase, and his wife Francesca, a ballet dancer; she is the youngest of three daughters. Her parents were both born Jewish, although her mother converted to Christianity when Julianna was five years old. Her parents' families were from Romania, Austria, Hungary, and Russia. As a child, Julianna lived in New York, England, and France.
- Is the only cast member of ER (1994) to have ever received an Emmy Award, plus she has been awarded the Screen Actors Guild Award for Outstanding Performance by a Female Actor in a Drama Series two years in a row (1998 and 1999).
- She was awarded a star on the Hollywood Walk of Fame for Television at 6621 Hollywood Boulevard in Hollywood, California. (May 1, 2015)
- Julianna's older sister, Alexandra Margulies, appeared as a flight attendant in her final series episode, Such Sweet Sorrow (2000).
- She turned down the role of Sasha Hassler in Face/Off (1997) because filming conflicted with her schedule for ER (1994). The role eventually went to Gina Gershon.
- She has starred on two series that are set in Chicago, Illinois: ER (1994) and The Good Wife (2009); both were filmed in Los Angeles and New York respectively.
- One of People magazine's 50 Most Beautiful People in the World (1998).
- Her favorite script-related app is called "Rehearsal 2", an iPad app that was introduced to her by Rita Wilson when she guested as Viola Walsh on The Good Wife (2009).
- She divides her time between SoHo, Manhattan, and Livingston, New York. (July 2008)
- When Julianna began work on The Good Wife (2009), her son Kieran was just 13 months old.
- Met her former boyfriend, Ron Eldard, in an acting class in 1991. They separated in 2003.
- In the movie Snakes on a Plane (2006), she played a flight attendant on her final flight before quitting to become a lawyer. Three years later, she started her run as lawyer Alicia Florrick on The Good Wife (2009).
- Counts actor-director Griffin Dunne as a close friend and confidant.
- Attended grade school at Green Meadow School in Spring Valley, New York, then High Mowing High School in Wilton, New Hampshire. She later received her Bachelor's degree in liberal arts from Sarah Lawrence College, where she majored in art history, spending her junior year abroad in Florence, Italy.
- Friends with George Clooney.
- Wedding dress was designed by Narciso Rodríguez.
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