Wile E. Coyote's pupils dilate, enveloping his irises, as he sees Roadrunner on a plate. The camera snaps to a closeup and his pupils are much smaller than the previous shot with the irises still not visible, and the pupils once again dilate.
When LeBron's family searches for him using GPS, it would not have led them directly to the server room in the basement as GPS can only accurately detect movement along latitude and longitude. While GPS can detect changes in altitude, it is much less accurate and off by up to about 23 meters in either direction about 95% of the time, and provides a relatively random result the remaining 5% of the time.
Wile E. Coyote's Acme multiplier allows him to score dozens of 3-pointers from across the court (viewers can clearly see the score increasing in intervals of 3), bringing the Tune Squad's score from 476 to an even 1000. This means Wile E. scored 524 points. There is also a +96 point bonus, but it is unclear if that is part of the 524 points or not. Regardless, neither 1000, nor 524, nor 428 (524-96) is divisible by 3, so the score has to be wrong somewhere.
When LeBron and Dom drop into the Warner 3000 Serververse, LeBron asks him if he's okay, and he says, "I'm fine, Dad.", but his lips never move.
When LeBron, Bugs and the rest of the Looney Tunes returned to Tune World, Lola wasn't seen when they were exiting Marvin's ship.