The full monster make-up was actually being worn by a man, Harry Wilson. Because of this, makeup creator Harry Thomas did not realize that the creature was supposed to be female. All he could do at the last minute was apply lipstick to the creature.
Sally Todd's scream at the beginning of the film is that of Allison Hayes from the soundtrack of Attack of the 50 Foot Woman (1958).
Director Richard E. Cunha recently recalled that, upon seeing the make-up for the title creature just before filming, he was so disappointed he left the set and broke down in tears.
Make-up artist Harry Thomas has said that when he was given the assignment to make a mask for the "monster", he was not told that the monster was to be a female, so he made the mask to be a male. By the time he was notified that the monster was to be a female, the mask had already been done, and there was no money in the make-up budget left to do another one. Thomas claims that director Richard E. Cunha's alleged statement giving Paul Stanhope credit for the make-up is false, as Thomas was the film's only make-up man. Thomas also discredits Cunha's claim that he wanted Jack P. Pierce to do the make-up, as Pierce was notorious for taking a long time to apply his creations. Thomas claims the gender problem of the monster would never have occurred if the filmmakers had bothered to give him a $2 copy of the script.
John Ashley had just made a number of films for American-International Pictures. He later recalled, "AIP was low budget --100 grand a movie--but at least they shot on sound stages and the size of the crew was bigger.' Frankenstein's Daughter' was really rock-bottom. But the people were very nice, especially Richard E. Cunha, the director . . . but it was quick, a little more down and dirty than AIP". Ashley later said he remembered two things about the film: "the monster, which was a man because the makeup artist didn't know it was supposed to be a woman, and that we shot the ending at Harold Lloyd's estate, because Harold Lloyd Jr. played a teenager in it".